Before creating a map, we should format our tileset for use. There are things to remember before you get started with Tiled.

• RPG Maker MV can only support 1024x1024 dimensions for tilesets max. This is really important and something you should not ignore. You do not have to make your tilesets 1024x1024. I would recommend to keep them smaller if you can. It is not the file size that causes loading issues on your game. It's the file dimensions and compression.
• RPG Maker Tilesets B, C, D, E work as is. There is no need to reformat them for the most part.
• You can have multiple tilesets per map and it isn't bound by layer. You don’t have to worry about running out of tilesets to use. Just don't go overboard for the sake of game performance.
• Once you decided on a grid size, you must use that size for the entire game. For example, you want to use 32x32 tiles and suddenly 48x48. You cannot do that.

I highly recommend to separate your tilesets based on their type and remove all duplicate tiles. RPG Maker tends to have those due to tileset editor limits (ABCDE). Since Tiled has no tileset limit, lessening the strain on your game project would benefit you in the long run.

Collision Tileset

Read Creating a Collision Tileset page.

Autotile Sheets

• RPG Maker VX-VXA follows a non-standard autotile format where Tiled's Terrain function will not work properly.
• We did not provide support for Tiled's Terrain function due to lack of graphical assets to test the function.
• There are currently no plans to provide Tiled Terrain support.

Therefore, you need to use the Autotile Converter and map the edges manually.

Animated Tileset

Read Setting Animated Tiles page.

Region Tileset

Read Creating a Region Tileset page.